VisionGroup

Antibody Purification

Antibody purification involves isolation of antibody from serum (polyclonal antibody), ascites fluid, or from the culture supernatant of a hybridoma cell line (monoclonal antibody). Purification methods range from very crude to highly specific. The level of purification necessary to obtain usable antibody depends upon the intended application(s) for the antibody. Antibodies may be purified by class (e.g., IgG) without regard to antigen specificity, or affinity purified to an immobilized antigen that is antibody specific.

We offer a wide selection of ligands and formats to purify total or specific antibodies from serum, ascites fluid, or tissue culture supernatant. Our portfolio is designed to meet small-scale (screening) to large-scale (bioprocess) needs.

Broad product selection—affinity supports for the purification and enrichment of multiple antibodies, including Protein A, Protein G, Protein A/G, and Protein L
More formats—magnetic beads, loose resin, spin columns and kits, FPLC cartridges, and 96-well filter plates enable antibody purification from microgram to kilogram scales
High performance—resins are designed to maximize protein yield and reduce background
Economical—pricing is similar to or better than our leading competitors

Choose the right antibody purification resin

	Protein A	Protein G	Protein A/G	Protein L	Melon Gel
Target	IgG enrichment	IgG enrichment	IgG enrichment	IgG enrichment	Negative selection
Use for	Polyclonal IgG from rabbit, pig, dog, cat serum	IgG from mouse, human, cow, goat and sheep	Polyclonal IgG from many species	Human or mouse monoclonal antibodies known to have appropriate kappa light chains	Antibodies that do not purify well using Protein A or Protein G
Formats	Magnetic beads, filter plates, loose resins (agarose and POROS), spin columns and kits, FPLC cartridges	Magnetic beads, filter plates, loose resins (agarose and POROS), spin columns and kits, FPLC cartridges	Magnetic beads, filter plates, loose resins (agarose and POROS), spin columns and kits, FPLC cartridges	Magnetic beads, filter plates, loose resins, spin columns and kits, FPLC cartridges	Serum and monoclonal antibody kits, FPLC cartridges, spin plate
Processing scale	µg–kg	µg–kg	µg–kg	µg–kg	µg–kg

Featured antibody purification categories

Antibody Purification Using Protein A

Protein A binds to human, mouse, goat, and rabbit IgG, (except for human IgG3 and mouse IgG1.) at the Fc portion of the intact Ig and is typically the ligand of choice when purifying IgG from rabbit polyclonal antisera.

Antibody Purification Using Protein G

Protein G binds all human, goat, and murine IgG and subclasses at the Fc portion of the intact Ig and is typically the ligand of choice for purifying IgG from goat or mouse polyclonal antisera.

Antibody Purification Using Protein A/G

Protein A/G is a genetically engineered protein with combined IgG binding profiles of both Protein A and G. It contains four Fc binding domains from Protein A and two from Protein G, providing additive class-specific binding properties for Protein A and G.

Antibody Purification Using Protein L

Protein L binds specifically to Ig kappa light chains, and members of all antibody classes (IgG, IgM, IgA, IgD, and IgE). It is the affinity molecule of choice for the positive selection of kappa Fab fragments or recombinant single-chain variable fragments.

Antigen-specific antibody purification

Choose from a variety of activated affinity supports (porous resins or magnetic beads) to successfully immobilize peptides, proteins, and other antigens, like amino, sulfhydryl, carboxyl, or glycosyl functional groups.

Large Scale Process Chromatography

When you need high performance and high throughput on a large scale, POROS™ bulk chromatography resins are the industry standard for process-scale bioseparations.

Immunoprecipitation

Dynabeads and other magnetic bead and resin products and technologies for IP, co-IP, pull-down, ChIP and other small-scale affinity purification and analysis assays.

General characteristics of Ig-binding proteins

	Native Protein A	Recombinant Protein A	Recombinant Protein G	Recombinant Protein A/G	Recombinant Protein L
Native source	Staphylococcus aureus	Staphylococcus aureus	Groups C and G Streptococci	N/A	Peptostrepto -coccus magnus
Production source	S. aureus	E. coli	E. coli	E. coli	E. coli
Molecular mass	46,700	44,600	21,600	50,460	35,800
Apparent mass by SDS-PAGE	42 kDa	45 kDa	32 kDa	40 to 45 kDa	36 kDa
Ig-binding sites (#)	5	5	2	4+2	4
Albumin binding site	No	No	No	No	No
Optimal binding pH	8.2	8.2	5	5 to 8.2	7.5
Ig binding target	Fc	Fc	Fc	Fc	VL-kappa

Related products for antibody purification

Catalog #	Name	Size	MSDS	Price (INR)
45206	Melon™ Gel IgG Spin Purification Kit	3 mL	-	51,017.00
45212	Melon™ Gel IgG Purification Kit	25 mL	-	106,981.00
45214	Melon™ Gel Monoclonal IgG Purification Kit	200 mL	-	234,796.00
45216	Pierce™ Saturated Ammonium Sulfate Solution	1 L	-	28,699.00
45219	Ascites Conditioning Reagent for Melon™ Gel Monoclonal IgG Purification Kit	5 mL	-	7,611.00
89932	Melon™ Gel Chromatography Cartridges, 1 mL	2 x 1 mL	-	33,180.00
89933	Melon™ Gel Chromatography Cartridges, 5 mL	1 x 5 mL	-	45,894.00
45208	Melon™ Gel Spin Plate Kit for IgG Screening	2 plates	-	65,309.00
89972	Melon™ Gel Purification Buffer	1 pack	-	5,314.00
89973	Melon™ Gel Regenerant	1 pack	-	5,355.00
44916	Pierce™ Thiophilic Adsorption Kit	4 columns	-	93,017.00
22212	Pierce™ Mannan Binding Protein Agarose	10 mL	-	199,845.00
44897	Pierce™ IgM Purification Kit	1 column	-	133,323.00
21017	Elution Buffer for Pierce™ IgM Purification Kit	500 mL	-	13,705.00
21018	MBP Column Preparation Buffer for Pierce™ IgM Purification Kit	50 mL	-	7,413.00
20395	Pierce™ Jacalin Agarose	5 mL	-	41,812.00

Bulk Quantities and Custom Products

Partner with us to scale-up your process or product development pipeline with bulk quantities of high-quality immunoassay reagents and biochemicals. We offer a variety of compounds, devices, and resins for your large-scale applications.