HLA-Ready Plate

HLA-Ready Plate

HLA-Ready Plate - microlymphocytotoxicity test
  • Thaw Ready Plates 10 - 15 minutes before use
  • Dispense 1 μl (2000 - 3000) lymphocytes into each well
  • Incubate for 30 min at room temperature (DR / DQ: 60 min.)
  • Thaw frozen rabbit complement or reconstitute lyophilized
  • After the end of the 30 min. Incubation time Add 5-6 μl rabbit composition and incubate for 60 min at room temperature (DR / DQ: 120 min.)
  • Add 3 - 4 μl eosin (5%), wait approx. 5 min., Then add 5-6 μl formalin (37% formaldehyde) for fixation
  • Cover with coverslip and read Ready Plates with the inverse phase contrast microscope.


The reaction strength is documented in score values

Score 1 0 - 10% Negative reaction
Score 2 11 - 20% Questionable negative reaction
Score 4 21 - 50% Questionable positive reaction
Score 6 51 - 80% Positive reaction
Score 8 81 - 100% Strong positive reaction
Score 0 no cells
item number Surname Test / plate
RUO Emergency for use in diagnostic procedures - USA and Canada
002 011 144 HLA-Ready Plate ABC 144 5
002 010 144 HLA-Ready Plate AB 144 5
002 011 120 HLA-Ready Plate ABC 120 5
002 010 120 HLA-Ready Plate AB 120 5
002 012 072 HLA Ready Plate ABC 72 10
002 014 072 HLA-Ready Plate AB 72 10
002 013 072 HLA-Ready Plate DR 72 10
002 021 001 HLA-Ready Plate B27 1 10
002 022 002 HLA-Ready Plate B27 2 20
002 023 005 HLA-Ready Plate B27 5 50
002 025 005 HLA-Ready Plate ABC Complement 5

item number Surname Test / plate
RUO Emergency for use in diagnostic procedures - USA and Canada
002 041 600 Lymphodex 6 x 100 ml
002 041 500 Lymphodex 500 ml
002 042 600 Hank's Solution 6 x 100 ml
002 042 500 Hank's Solution 500 ml
002 043 500 RPMI-1640 500 ml
002 044 005 Rabbit Complement 5 x 1 ml
002 044 005L Lyophilized Rabbit Complement 5 x 1 ml
002 045 001 HLA Control positive 0.5 ml
002 045 002 HLA Control negative 0.5 ml
002 047 500 Lymphofix 500 ml