Cloning



Get started with cloning


We offer powerful and versatile Invitrogen cloning and expression vectors, GeneArt Gene Synthesis and assembly tools, and molecular biology essentials for that critical first step in your experiment. Unsure where to start? Find out more >

Featured cloning categories

TOPO Cloning

5-minute, room-temperature cloning with no gel purification or post-PCR modifications & up to 95% recombinants with TOPO cloning.

Gene Synthesis

Combining the convenience of the Invitrogen online ordering system, with cutting-edge GeneArt service offerings.

Blunt-End Cloning

High-efficiency cloning of blunt-ends using Invitrogen Zero Blunt cloning kits.

GeneArt Type IIs Assembly

Based on Type IIs restriction enzymes for non-recombination-based cloning of multiple DNA inserts.

Long Fragment Cloning

Easily clone long fragments using the TOPO XL-2 Complete PCR Cloning Kit.

Gateway Cloning

Gateway recombination cloning technology allows gene shuttling, enabling you to access virtually any expression system.

TA Cloning

TA cloning technology relies on the complimentary of thymine & adenine. Enhanced with Invitrogen ExpressLink T4 DNA Ligase.

Competent Cells

Choose competent cells based on application, strain, packaging format & transformation efficiency.

Restriction Enzyme Digestion & Ligation

Browse our new one-buffer Anza restriction enzyme cloning system as well as our Invitrogen conventional restriction and modifying enzymes.

PCR Enzymes & Reagents

PCR enzymes for any application: high fidelity, high specificity, long fragment, GC-rich, genotyping, or routine PCR.

Oligos

A variety of scales, formats, modifications, & purifications to meet your cloning needs.

Vector NTI Software

Industry-leading sequence analysis & data management software

GeneArt Seamless Cloning & Genetic Assembly

Clone up to 10 DNA fragments simultaneously; assemble existing fragments without restriction digest or PCR amplification.

Thermo Scientific Cloning Tools

Thermo Scientific product line such as FastDigest restriction enzymes, CloneJET kits, InsTAclone kits, Ligase Independent Cloning, Alkaline Phosphatases and more.

Synthetic Biology

Find tools, kits, and services for synthetic gene production, including custom-made linear fragments, solutions for genetic assembly and mutagenesis, and online ordering of optimized genes cloned into the vector of your choice.

Molecular Biology Workflow Management

Track and manage data throughout the therapeutic discovery and development processes.

Resources
Cloning Support Center

Find tips, troubleshooting help, and resources for your cloning applications.

Cloning services

We offer everything from custom vector design to high-throughput protein expression.

Molecular Cloning Education

Learn about the foundations of recombinant DNA technology

Tools